RTG-2
ACC 352
Cell line:
RTG-2
DSMZ no.:
ACC 352
Species:
fish - rainbow trout (Oncorhynchus mykiss)
Cell type:
gonadal cells
Origin:
established from the gonadal cells of a rainbow trout (salmo gairdneri)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% MEM (with Earle's salts) + 10% h.i. FBS + 2 mM L-glutamine + 50 mg/L neomycin sulfate
Subculture:
split subconfluent culture 1:2 every 5-7 days using trypsin/EDTA at cold temperature; seed out at ca. 0.5 x 106 cells/25 cm2; growth is inhibited at a pH higher than 7.4
Incubation:
maximum temperature at 26 °C, optimal at 20 °C, grown with CO2
Doubling time:
ca. 3-7 days
Harvest:
cell harvest of ca. 4 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization and PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Oncorhynchus mykiss species.
Viruses:
PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list