HH-16.cl.4
ACC 358
Cell line:
HH-16.cl.4
DSMZ no.:
ACC 358
Species:
rat (Rattus norvegicus)
Cell type:
mammary tumor
Origin:
established from the ascitic fluid of a Sprague-Dawley rat (adult female, 240-days-old) with a rat mammary tumor in 1977; tumor was produced by injection of cultured cells from DMBA-induced mammary tumor (DMBA = 9,10-di-methyl-1,2-benzanthracene); derived from identical tumor as HH-16 cl.2/1 (DSM ACC 485); cells were described in the literature to produce invasive adenocarcinomas in S.D. rats upon s.c. injection with remote metastases in lymph nodes and lung
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate
Subculture:
split confluent culture 1:5 to 1:10 twice a week using trypsin/EDTA; seed out at ca. 2-3 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-80 hours
Harvest:
cell harvest of ca. 1.0-1.5 x 105 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Cytogenetics:
rat flat-moded near-tetraploid karyotype with 10% polyploidy - 79-84<4n>
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list