SD-1
ACC 366
Cell line:
SD-1
DSMZ no.:
ACC 366
Species:
human (Homo sapiens)
Cell type:
peripheral blood B lymphoblastoid cells (from patient with acute lymphoblastic leukemia)
Origin:
established from the peripheral blood of a woman with acute lymphoblastic leukemia (pre B-ALL) at diagnosis; cells were immortalized with EBV; shown at DSMZ by RT-PCR to carry minor breakpoint BCR-ABL1 fusion for which it is a suitable positive control for RT-PCR
Biosafety level:
1
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects.
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
85-90% RPMI 1640 + 5-10% h.i. FBS
Subculture:
seed out at ca. 0.3 x 106 cells/ml; maintain at 0.3-1.0 x 106 cells/ml; split cells 1:3 to 1:5 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 28-32 hours
Harvest:
reaches maximum density of ca. 1.3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD37 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, sm/cylambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-tetraploid karyotype - 92(88-92)<4n>XXXX, t(9;22)(q34;q11)x2 - carries two balanced Ph translocations - tetraploid derivate of original diploid karyotype
Molec. Genetics:
expression of fusion gene BCR-ABL1 (e1-a2) confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list