LCLC-103H
ACC 384
Cell line:
LCLC-103H
DSMZ no.:
ACC 384
Species:
human (Homo sapiens)
Cell type:
large cell lung carcinoma
Origin:
established from the pleural effusion of a 61-year-old man with large cell lung carcinoma with giant cells who had received chemo- and radiotherapy; described in the literature to be PAS negative, to exhibit remarkable stroma formation and to overexpress the proto-oncogene MYC; listed in the CCLE (ref 18232)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2; split confluent cultures 1:2 to 1:6 using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40 hours
Harvest:
cell harvest of ca. 9-13 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
originally tested as negative in DAPI, microbiological culture, RNA hybridization, and recently by PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human, highly rearranged flat-moded hexaploid/heptaploid karyotype with 4% polyploidy - 130-160<7n>XXY, -X, -Y, -Y, +1, +1, -2, -2, -3, -6, -6, -8, -8, -9, -9, -9, -10, -13, -13, -13, -14, -14, +15, +16, +16, -17, -18, -19, -19, -20, -20, -20, -20, +18-25mar, del(1)(p22.2), add(1)(q11-21), i(1p)x2, add(3)(p23-24)x2, del(5)(q11q15), i(5q)x2, add(5)(p?), add(6)(q11)x2-3, add(7)(q34)x2, del(7)(q22)x2, i(8q), del(9)(p21)x2-3, del(10)(q23-24)x2, der(10;13)(q10;q10)x2, add(11)(q24-25)x2, del(11)(q23)x2, add(13)(p11), add(16)(q24) - variable number of dmin present - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list