
HT-1376
ACC 397
Cell line:
HT-1376
DSMZ no.:
ACC 397
Species:
human (Homo sapiens)
Cell type:
urinary bladder carcinoma
Origin:
established from a transurethral resection of invasive, moderately pleomorphic (grade 3) bladder transitional cell carcinoma of a 58-year-old woman who had not received chemo- or radiotherapy
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
85% DMEM (4.5 g/L glucose) + 15% h.i. FBS + 2 mM L-glutamine
Subculture:
split confluent cultures 1:5 once a week using trypsin/EDTA; seed out at ca. 1.5 x 106 cells/80 cm2; maximal density at ca. 60 x 106 cells/175 cm2
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 60 hours
Harvest:
cell harvest of ca. 40 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded pentaploid karyotype with 8% polyploidy - 111(102-116)<5n>XX/XXX, +3, +3, +5, +5, -6, +7, +8, +8, -12, -13, -13, -14, -14, -15, -15, -15, -15, -16, -17,-17, -19, -19, +20, -22, -22, -22, +6-8mar - del(2)(q25), add(3)(q27)x2, del(3)(p11p14), i(5q), add(5)(p15), del(5)(p13), add(5)(q1?3;hsr), del(6)(q25), add(7)(p22), del(7)(q11), add(8)(p23)x2, i(9q)x2, del(9)(p21), del(10)(q24), add(11)(p15), add(12)(p13)x1-2, del(12)(p11), der(13)t(13;?)(p11;?)t(?;1)(?;q11), add(17)(p1?), add(18)(p11) - sideline with i(5p) - 3p, 5p/q10, +7, 8p, 9p, 10q, 11p, 17p rearrangements associated with bladder TCC - matches published karyotypes
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list