FDCP-Mix cl.A4
ACC 401
Cell line:
FDCP-Mix cl.A4
DSMZ no.:
ACC 401
Species:
murine (mouse) (Mus musculus)
Cell type:
bone marrow
Origin:
established from the long-term bone marrow cultures of BDF1 mice; cells are mIL-3-dependent (FDCP means "Factor-Dependent Continuous cell lines, Paterson Laboratories" after the Paterson Institute, Manchester, UK); it is described that cells can be maintained in a primitive state or induced to differentiate
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
round, single cells in suspension, sometimes in small clusters
Medium:
80% Iscove's MDM + 20% h.i. FBS + 10 ng/ml murine IL-3 (or 10-20% conditioned medium of cell line WEHI-3B, DSM ACC 26)
Subculture:
split saturated culture 1:2 every 3-4 days; seed out at ca. 1.0 x 106 cells/ml; after thawing, cells are initially very difficult to culture and viability may be initially rather low (20-30%): hence it is advisable to culture the cells first in 24-well-plates and to exchange the medium, adding fresh mIL-3, already about 5-8 hours after thawing and then daily. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines.
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 70-80 hours
Harvest:
maximum density at about 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine near-diploid karyotype with 10% polyploidy - 40(38-40)<2n> - fusion markers absent
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list