MKN-45

human (Homo sapiens)

gastric adenocarcinoma

established from the poorly differentiated adenocarcinoma of the stomach (medullary type) of a 62-year-old woman

14790, 14795, 14886

1

A

both spindle-shaped or oval cells growing in monolayers and single round cells or clumps in suspension; image; image; image

80% RPMI 1640 + 20% h.i. FBS

cells can be difficult to culture; seed out at ca. 1-2 x 10⁶ cells/80 cm²; split semi-confluent culture 1:3 to 1:5 every 5-10 days using trypsin/EDTA (for 5 min or longer); culture medium may turn acidic (yellow) very quickly, exchange of spent medium is advised, also the cells in suspension may be used to seed a new flask

at 37 °C with 5% CO₂

ca. 60 hours

cell harvest of ca. 10-15 x 10⁶ cells/80 cm²

frozen with 70% medium, 20% FBS, 10% DMSO

initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

human hypertriploid karyotype with 8% polyploidy - 70-73<3n>XX/XXX, -X, -2, -2, -4, -5, -5, +6, -11, -13, -13, -14, -18, -19, -20, -21, -22, +20mar, add(X)(q1?), der(1)(1pter->p21::1p11->q32::1q44->q11::?), i(2p), der(3)t(3;?9)(p14;q13/21), del(4)(q21), del(4)(q26), del(6)(q1?)x2, del(7)(p11), del(8)(p12), add(8)(q24), del(9)(p11), add(13)(p11), inv(16)(p13q22), add(17)(p1?), add(19)(q13), i(21q) - resembles published karyotype

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Delivery form		Prices
Frozen culture		450,- €
Growing culture   (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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