C7
ACC 416
Cell line:
C7
DSMZ no.:
ACC 416
Species:
murine (mouse) (Mus musculus)
Cell type:
macrophages
Origin:
established from the bone marrow of male adult 129/Sv mice (p53 gene knocked out); it is described that the differentiation potential of C7 cells to osteoclasts is maintained in the presence of M-CSF and is abolished irreversibly by the stimuli known to induce differentiation into other hematopoietic lineages
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10% h.i. FBS + 1 ng/ml M-CSF (human or murine)
Subculture:
split confluent culture 1:2 to 1:4 every 4-6 days using trypsin/EDTA after washing twice with PBS; seed out at ca. 0.5 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 80-100 hours
Harvest:
cell harvest of ca. 2-3 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypertriploid karyotype with 20% polyploidy - 66(64-67)<3n> - no centric fusion markers present
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list