MFE-296
ACC 419
Cell line:
MFE-296
DSMZ no.:
ACC 419
Species:
human (Homo sapiens)
Cell type:
endometrium adenocarcinoma
Origin:
established from the primary tumor of a 68-year-old woman with moderately differentiated endometrial adenocarcinoma (grade G2) in 1991; cells were described in the literature to express androgen receptors (androgen was reported to inhibit cell proliferation), but no estrogen receptors; cells were described in the literature to be tumorigenic in nude mice
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
40% RPMI 1640 + 40% MEM (with Earle'salts) + 20% h.i. FBS + 2 mM L-glutamine + 1x insulin-transferrin-sodium selenite
Subculture:
seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:5 once or twice a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-60 hours
Harvest:
cell harvest of ca. 10 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human diploid karyotype with 20% polyploidy - 46<2n>XX, no consistent abnormality detected - matches published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/I I-, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list