
KG-1a
ACC 421
Cell line:
KG-1a
DSMZ no.:
ACC 421
Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia (derivative of KG-1)
Origin:
established from the bone marrow of a 59-year-old man with erythroleukemia that developed into acute myeloid leukemia (AML); KG-1a is a subclone of AML cell line KG-1 (DSM ACC 14) described as "undifferentiated variant" not responsive to CSF
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80% alpha-MEM (with ribo- and deoxyribonucleosides) or RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 1.0 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; split 1:2 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
maximum density at ca. 2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD4 -, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-diploid karyotype with 5% polyploidy - 46(44-47)<2n>X/XY, +8, +11, -12, -17, -20, der(5;17)(q10;q10)del(5)(q?11q?13), del(7)(q22q35), i(8q), der(8)t(8;12)(p11;q13)add(8)(q24), i(11q), add(16)(q13/21), der(19)t(14;19)(q11;q13), add(20)(p13) - sideline with -18 - resembles published karyotype - closely related to, but distinct from KG-1
Molec. Genetics:
expression of fusion gene FGFR1OP2-FGFR1 (OP2-FGFR1) confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list