MFE-319
ACC 423
Cell line:
MFE-319
DSMZ no.:
ACC 423
Species:
human (Homo sapiens)
Cell type:
endometrium adenocarcinoma
Origin:
established from the primary adenosquamous endometrium carcinoma (grading G1-G2) of an 81-year-old woman in 1992; described in the literature to form poorly differentiated tumors in nude mice
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
40% RPMI 1640 + 40% MEM (with Earle's salts) + 20% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/25 cm2; split confluent culture 1:2 to 1:3 every 5 days using trypsin/EDTA (cells are difficult to detach from the flask; thoroughly washing with PBS and incubation with trypsin/EDTA of 15 min or longer at 37 °C is recommended)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 70-90 hours
Harvest:
cell harvest of ca. 15-20 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
originally negative in DAPI, microbiological culture, RNA hybridization, and continuously by PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypodiploid karyotype with 4% polyploidy - 45(43-46)<2n>X, -X, t(13;18)(q14.3-21;q11-12) - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list