D3
ACC 428
Cell line:
D3
DSMZ no.:
ACC 428
Species:
murine (mouse) (Mus musculus)
Cell type:
hybridoma (anti-chicken N-CAM)
Origin:
cell line was established by fusion of mouse NS-1 cells with spleen cells from a BALB/c mouse immunized with wheat germ agglutinin affinity purified chicken brain proteins immobilized on lectin beads; described as secreting a mouse monoclonal antibody (IgG) reacting with the cytoplasmic domain of the 140, 180 and 250 kDa glycosylated neuronal cell surface protein N-CAM, preferentially in neurons of the chicken visual system like retina, tectum and other CNS regions and PNS; also reacting with mouse, goldfish and human N-CAM in paraformaldehyde-fixed cryosections and Western blots
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
round, single cells growing in suspension and some slightly adherent
Medium:
90% RPMI 1640 + 10% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement")
Subculture:
maintain at ca. 0.5 x 106 cells/ml; split saturated culture 1:5 to 1:10 every 2-3 days (some cells attach to the surface of the culture flask, but can be detached by tapping the flask); seed out at ca. 0.5 x 106 cells/ml; maximum density of ca. 1-2 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 22 hours
Harvest:
cell harvest of ca. 0.5-1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
secretion of a IgG2A monoclonal antibody
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine flat-moded hypertetraploid karyotype - 98-104<4n> - two centric fusion markers present
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list