VLM
ACC 429
Cell line:
VLM
DSMZ no.:
ACC 429
Species:
murine (mouse) (Mus musculus)
Cell type:
SV40-transformed embryo cells
Origin:
established from SV40-transformed BALB/c mouse embryo cells; cells are described as possessing SV40 T-antigen and tumor-specific transplantation antigens and as not releasing spontaneously SV40; tumorigenic in mice
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
adherent fibroblastoid cells growing in monolayers
Medium:
80-90% DMEM + 10-20% h.i. FBS
Subculture:
split confluent culture 1:3 to 1:6 every 3-6 days using trypsin/EDTA; seed out at ca. 2-3 x 105 cells/ml in 10 ml medium;
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 25 hours
Harvest:
cell harvest of maximal 13 x 106 cells/25 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypertriploid karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list