X63AG8.653
ACC 43
Cell line:
X63AG8.653
DSMZ no.:
ACC 43
Species:
murine (mouse) (Mus musculus)
Cell type:
myeloma
Origin:
X63AG8.653 is a non-secreting clone derived from P3X63AG8 (a Balb/c myeloma) and is recommended as fusion parent for production of hybridomas
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at about 1 x 106 cells/ml; maintain at 0.1-1.0 x 106 cells/ml; split culture in the logarithmic phase 1:10 to 1:20 every 48 hours; cultivate initially with 20% FBS
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 30-50 hours
Harvest:
maximal density of ca. 1.5-2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list