MS-5
ACC 441
Cell line:
MS-5
DSMZ no.:
ACC 441
Species:
murine (mouse) (Mus musculus)
Cell type:
stromal cells
Origin:
established by irradiation of the adherent cells in long-term bone marrow cultures derived from C3H/HeNSlc strain mice
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10% horse serum (or h.i. FBS) + 2 mM L-glutamine + 2 mM sodium pyruvate
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:2 to 1:3 every third day using trypsin/EDTA; maximal density at ca. 15 x 106 cells/175 cm2; CAVEAT: cells may detach spontaneously in aggregates which are difficult to disperse
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40 hours
Harvest:
cell harvest of ca. 10-15 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypertriploid karyotype
Viruses:
PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list