AC-1M32
ACC 442
Cell line:
AC-1M32
DSMZ no.:
ACC 442
Species:
human (Homo sapiens)
Cell type:
choriocarcinoma-trophoblast hybrid
Origin:
one of five phenotypically and cytogenetically distinct subclones of a hybrid (ACH1P, DSM ACC 481) formed by fusing an HGPRT-defective mutant (AC1-1) of JEG-3 human choriocarcinoma (DSM ACC 463) with primary human trophoblast (chorion laeve) cells
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
epitheloid cells growing adherently in monolayers
Medium:
90% Ham's F12 + 10% h.i. FBS
Subculture:
split confluent culture 1:3 to 1:6 every 3-5 days using trypsin/EDTA; seed out at ca. 0.1-0.2 x 105 cells/cm2; maintain at ca. 0.1-1.3 x 105 cells/cm2; reaches max. density of ca. 1.8 x 105 cells/cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 20-30 hours
Harvest:
cell harvest of ca. 1.0-1.5 x 105 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, RNA hybridization and continuously in PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
near identical DNA profiles shared by the AC-sister cell lines, partly identical with JEG-3 and BEWO
Cytogenetics:
human flat-moded hypertetraploid karyotype with 4% polyploidy - 80-104<4n>XXXY, +1, +2, +3, -4, +7, +9, -13, -13, -15, +16, +16, -18, -20, +21, +22, +5-7mar, del(X)(p11p22), der(X)(Xpter->Xq2?8::4p1?->4p1?::11q1?1->11qter), del(1)(p11), der(7)t(7;7)(p22;q11.22)add(7)(q32), add(7)(q32), add(9)(p24), add(11)(p15), i(13q), add(15)(p13), add(19)(p13) - sideline with: idem, +7, +16, -18, del(1)(p11), +del(1)(p22) - resembles published karyotype and those of sisters with minor, though consistent, differences (mirroring DNA profiles)
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list