BC-3C
ACC 450
Cell line:
BC-3C
DSMZ no.:
ACC 450
Species:
human (Homo sapiens)
Cell type:
urinary bladder transitional cell carcinoma
Origin:
derived from the invasive solid transitional cell carcinoma of the bladder of a 82-year-old woman (grade IV, stage C); cell growth described as being inhibited by EGF
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% McCoy's 5A + 10% h.i. FBS
Subculture:
seed out at ca. 1-3 x 106 cells in a T75 flask; recover and initially culture cells in 20% FBS; split confluent culture up to 1:10 every 3-7 days using trypsin/EDTA; maximum density of ca. 3.5 x 105 cells/cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 25-30 hours
Harvest:
cell harvest of ca. 2-3 x 105 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, RNA hybridization and continuously in PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypertriploid karyotype with 10% polyploidy - 65-72<3n>XX, -X, -2, -4, +9, +9, -11, -13, +14, +16, -17, -17, -18, +19, +20, +20, +2-3mar, add(2)(p1?), del(3)(q2?2), der(5;7)(q10;q10), del(9)(p2?2p2?3), add(12)(q24) - sideline with del(2)(q2?), i(3q), add(7)(p22), add(8)(p12), add(11)(q22-24), i(?17p), i(17q) and extra numerical changes - matches published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list