RO
ACC 452
Cell line:
RO
DSMZ no.:
ACC 452
Species:
human (Homo sapiens)
Cell type:
B lymphoblastoid cells (from patient with severe combined immunodeficiency, SCID)
Origin:
established from the peripheral blood of a 3-year-old boy with severe combined immunodeficiency (SCID) by EBV-transformation using the cell line B95-8 as source for EBV; cells were described as lacking MHC class II molecules
Biosafety level:
1
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects.
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
split saturated culture at 1:2 every two days; seed out at ca. 1.0 x 106 cells/ml; it is advisable to culture the cells initially in 24-well-plates; cell growth may be slow for the first week maximal density at ca. 2.0 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-50 hours
Harvest:
cell harvest of ca. 1.0-1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, CD138 -, HLA-DR -, cyIgG -, cyIgM +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-diploid karyotype with 8% polyploidy - 46(43-46)<2n>XY, no consistent abnormality present
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list