AC-1M81
ACC 456
Cell line:
AC-1M81
DSMZ no.:
ACC 456
Species:
human (Homo sapiens)
Cell type:
choriocarcinoma-trophoblast hybrid
Origin:
one of five phenotypically and cytogenetically distinct subclones of a hybrid (ACH1P, DSMZ ACC 481) formed by fusion of the JEG-3-derived HGPRT-defective mutant cell line AC1-1 with primary male trophoblast cells isolated from term human chorion laeve; described as possessing both, a distinct karyotype and a distinct expression pattern of tumor-related genes
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% Ham's F12 + 10% h.i. FBS
Subculture:
split confluent culture 1:3 to 1:5 twice a week using trypsin/EDTA; seed out at ca. 2-3 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 24 hours
Harvest:
cell harvest of ca. 0.4 x 106 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with sparfloxacin, then negative in microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
near identical DNA profiles shared by the AC-sister cell lines, partly identical with JEG-3 and BEWO
Cytogenetics:
human flat-moded hypertetraploid karyotype with 4% polyploidy - 80-106<4n>XX, -Y, -3, -4, +7, +9, -13, -18, +22, +4-6mar, del(X)(p11p22), der(X)(Xpter->Xq2?8::4p1?->4p1?::11q1?1->11qter), der(7)t(7;7)(p22;q11.22)add(7)(q32), add(7)(q32)x2, i(13q), add(15)(p13), add(19)(p13), der(19)t(11;19)(q11;q13) - resembles published karyotype and those of sisters with minor, though consistent, differences (mirroring DNA profiles)
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list