AC-1M88
ACC 457
Cell line:
AC-1M88
DSMZ no.:
ACC 457
Species:
human (Homo sapiens)
Cell type:
choriocarcinoma-trophoblast hybrid
Origin:
one of five phenotypically and cytogenetically distinct subclones of a hybrid (ACH1P, DSMZ ACC 481) formed by fusing an HGPRT-defective mutant (AC1-1) of JEG-3 human choriocarcinoma (DSMZ ACC 463) with primary human trophoblast (chorion laeve) cells
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% Ham's F12 + 10% h.i. FBS
Subculture:
split confluent culture 1:3 to 1:6 every 3-5 days using trypsin/EDTA; seed out at ca. 0.1-0.2 x 105 cells/cm2; maintain at ca. 0.1-0.5 x 105 cells/cm2; reaches max. density of ca. 1.3 x 105 cells/cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-50 hours
Harvest:
cell harvest of ca. 0.9 x 105 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, RNA hybridization, and PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database and identical with the other AC-1 sublines
Cytogenetics:
human flat-moded hypertetraploid karyotype with 11% polyploidy - 80-99<4n>XXX, -Y, -3, +7, +7, -8, +9, +9, +12, -13, +3-5mar, del(X)(p11p22), der(7)t(7;7)(p22;q11.22)add(7)(q32)x2, add(7)(q32), add(9)(p24), i(13q), add(19)(p13), der(19)t(11;19)(q11;q13) - sideline with idem, +1, +7, +12, der(4)t(4;11)(p14-16;q1?3) - resembles published karyotype and those of sisters with minor, though consistent, differences (mirroring DNA profiles)
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list