VIP-VIIIC8
ACC 479
Cell line:
VIP-VIIIC8
DSMZ no.:
ACC 479
Species:
murine (mouse) (Mus musculus)
Cell type:
hybridoma (CD2)
Origin:
described as secreting a mouse monoclonal antibody (IgG2a) CD2; the cell line was established in 1987 by fusion of PX63AG8.653 cells with spleen cells of a mouse immunized with fresh peripheral blood lymphocytes from patients with expansions of gamma-delta Tcells
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
single round cells growing in suspension
Medium:
80% DMEM (4.5 g/L glucose) + 20% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement")
Subculture:
split saturated cultures 1:2 to 1:3 every 3 days; seed out at 0.8 x 106 cell/ml, best in 12-well-plate with HFCS; maintain at 0.3-0.5 x 106 cells/ml; some cells attach to the surface of the culture flask, but can be detached by tapping the flask; for the first 2 weeks, HFCS should be used which thereafter can be reduced to 50% or 25% of the recommended concentration; cells grow very slowly in the complete absence of HFCS
Incubation:
at 37°C with 10% CO2
Doubling time:
about 40-50 hours
Harvest:
maximal density of about 1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
secretion of a IgG2a monoclonal antibody
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list