ACH1P
ACC 481
Cell line:
ACH1P
DSMZ no.:
ACC 481
Species:
human (Homo sapiens)
Cell type:
choriocarcinoma-trophoblast hybrid
Origin:
established by somatic hybridization of primary male trophoblast cells and the HGPRT-defective choriocarcinoma cell line AC1-1 which was originally derived from cell line JEG-3 (DSM ACC 463)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
adherent epithelial-like cells growing as monolayers, cells carry a large number of dark granula
Medium:
90% Ham's F12 + 10% h.i. FBS
Subculture:
split confluent culture 1:4 every 4-5 days week using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-50 hours
Harvest:
cell harvest of ca. 8 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with ciprofloxacin, then negative in microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypertetraploid karyotype with 10% polyploidy - 85-104<4n>XXY/XXYY/XXXY, +2, +5, +7, -10, -11, +12, -13, +16, -18, +21, +1-4 mar, add(7)(q32), i(13q), add(15)(p11) add(19)(p13)x1-2 - sideline with del(X)(p11p22), der(X)(Xpter->Xq2?8::4p1? ->4p1?::11q1?1->11qter), der(4)t(4;11)(p14-16;q1?3), der(7)t(7;7)(p22;q11.22), add(11)(p15) - karyotype closely resembles AC-1M32/46/59/81/88 (daughters) and JEG-3 (parent)
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list