MMQ
ACC 484
Cell line:
MMQ
DSMZ no.:
ACC 484
Species:
rat (Rattus norvegicus)
Cell type:
pituitary tumor
Origin:
cell line is derived from the transplantable 7315a rat pituitary tumor; until establishment of the cell line, the 7315a pituitary tumor was serially transplanted in adult female Buffalo rats since the early 1960s; described as expressing prolactin and dopamine receptors; cells were reported to be responsive to somatostatin, estrogen, progesterone, and acetylcholin
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
spheroidal cells growing in clumps in suspension
Medium:
90% RPMI 1640 + 7.5% horse serum + 2.5% h.i. FBS
Subculture:
optimal split ratio of 1:2 to 1:4 every 2-4 days; seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-1.0 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48 hours
Harvest:
maximum density at 1.5-2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 15% horse serum, 5% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Cytogenetics:
rat hyperdiploid karyotype with 6% polyploidy - 49-52<2n> - high level of spontaneous breakage
Viruses:
PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list