HH-16 cl.2/1
ACC 485
Cell line:
HH-16 cl.2/1
DSMZ no.:
ACC 485
Species:
rat (Rattus norvegicus)
Cell type:
mammary fibrosarcoma
Origin:
established from the ascitic fluid of a rat with a tumor which was produced by injecting cultured cells of a chemically-induced (dimethylbenzanthracene) mammary tumor; rat: strain Sprague-Dawley, female, 240 days old, adult; the original tumor was induced by single feeding of DMBA on day 45 after birth; sister cell line of HH-16 cl. 4 (DSM ACC 358); cells were described in the literature to produce invasive fibrocarcinomas in S.D. rats upon s.c. injection with remote metastases
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
fibroblastoid growing in a criss-cross pattern
Medium:
90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine
Subculture:
split confluent culture 1:4 every 3-4 days using trypsin/EDTA (NB: cells are sensitive to trypsin/EDTA and should not be incubated for more than 5 min at room temperature); seed out at ca. 1-2 x 106 cells/80 cm2; maintain at 4-5 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40 hours
Harvest:
cell harvest of ca. 7-8 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Cytogenetics:
rat near diploid karyotype with 11% polyploidy - 39-43<2n>
Viruses:
PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list