PR-1
ACC 486
Cell line:
PR-1
DSMZ no.:
ACC 486
Species:
rat (Rattus norvegicus)
Cell type:
pituitary tumor
Origin:
established from the pituitary tumor of an ovarectomized F344 rat treated with estrogen for 3 months; described as showing markedly reduced levels of TGF-ß1 and TGF-ß type II receptor mRNA; reported as being resistant to antiproliferative effects of TGF-ß1
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
round cells, slightly adherent, growing as strings of round cells in branched colonies
Medium:
DMEM + Ham's F12 (1:1) + 2.5% h.i. FBS + 10% horse serum
Subculture:
optimal split ratio of 1:2 once a week using trypsin/EDTA; seed out at ca. 3 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 90 hours
Harvest:
cell harvest of ca. 50 x 106 cells/80 cm2
Storage:
frozen with 65% medium (1:1), 5% FBS, 20% horse serum, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Cytogenetics:
rat near-triploid karyotype with 10% polyploidy - 60-68<3n>
Viruses:
PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list