Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established from the peritoneal effusion of a 38-year-old man with B-NHL (diffuse large cell, cleaved cell type; originally described as "diffuse histiocytic lymphoma") in 1975; cell line carries EZH2 Y641S mutation; assigned to GCB-like lymphoma subtype (germinal center B-cell). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.5-1.0 x 106 cells/ml; after thawing, during the first week cells should be kept with 20% FBS; culture in 12- or 24-well-plate is of advantage; split saturated culture 1:2 to 1:3 every 2-3 days; maintain at about 0.5-1.5 x 106 cells/ml; take care: viability may also decrease over the weekend when diluted too weak
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40 hours
Harvest:
cell harvest of about 1.0-2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, sm/cylambda -;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 4% polyploidy - 50(47-51)<2n>XY/XXY, -3, +7, +8, +12, +13, -16, +18, der(3)t(3;3)(p2?4;p1?2), der(8)t(3;8)(p1?2;q12)?inv(3)(p1?p2?), der(12)t(3;12)(p1?;q24)add(3)(p2?4), add(13)(p11), t(14;18)(q32.3;q21.1), add(16)(p13), der(18)t(14;18)(q32.3;q21.1) - carries t(14;18) effecting BCL2(MBR)-IGH fusion - matches published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
