KCI-MOH1 - Discontinued
ACC 498
Cell line:
KCI-MOH1 - Discontinued
DSMZ no.:
ACC 498
Species:
human (Homo sapiens)
Cell type:
pancreas adenocarcinoma (cell line HPAC)
Origin:
Caution: Cytogenetic and STR analysis showed that KCI-MOH1 is genetically identical to the human pancreas adenocarcinoma cell line HPAC.
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
adherent epithelial-like cells growing in monolayers
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
split confluent culture 1:2 to 1:3 every 3-4 days using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm2; maintain at ca. 3-4 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 70 hours
Harvest:
maximal density at ca. 7 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with enrofloxacin (Baytril), then negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis resulted in the STR profile of cell line HPAC.
Cytogenetics:
human hypotriploid karyotype with 8% polyploidy - 61(57-63)<3n>X/XX, -Y, +1, -2, -6, +8, -10, +11, -12, -13, -14, -16, -18, -21, -21, +mar, add(8)(p2 ?)x2, i(11p), del(11)(p12), add(13)(p11), add(17)(p12) - differs from published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as: