RS4;11
ACC 508
Cell line:
RS4;11
DSMZ no.:
ACC 508
Species:
human (Homo sapiens)
Cell type:
B cell precursor leukemia
Origin:
established from the bone marrow of a 32-year-old woman with acute lymphoblastic leukemia (ALL L2) in first relapse
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.3-0.5 x 106 cells/ml; maintain at about 0.5-1.0 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
maximal density of about 4.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 -, CD13 -, CD19 +, CD20 -, CD34 -, CD38 +, CD80 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype - 47/48<2n>X/XX, +8, +18, t(4;11)(q21;q23), i(7q) - sideline with +8/18 - carries KMT2A-AFF1 (MLL-AFF1; MLL-AF4) fusion gene - resembles published karyotype
Molec. Genetics:
expression of fusion gene KMT2A-AFF1 (MLL-AFF1; MLL-AF4) e10-e4 confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list