SEWA
ACC 523
Cell line:
SEWA
DSMZ no.:
ACC 523
Species:
murine (mouse) (Mus musculus)
Cell type:
osteogenic sarcoma
Origin:
established from a polyoma virus-induced osteogenic sarcoma in an A.SW mouse; cells were converted into ascitic form growing in suspension by serial intraperitoneal inoculation into genetically compatible mice; described in the literature to have lost double minute chromosomes which were still present in the original tumor cell line, but reoccur when retransplanted into mice
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90-95% RPMI 1640 + 5-10% h.i. FBS
Subculture:
split saturated culture 1:4 to 1:10 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.3-1.5 x 106 cells/ml; maximal density of ca. 2.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 20 hours
Harvest:
cell harvest of ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine flat-moded near-diploid karyotype - 36-44<2n> - dmin rare or absent
Viruses:
PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Growing culture (please inquire for exact delivery time) | 1040,- € | |
| Frozen culture | 520,- € | |
| DNA isolated from cell line (25 µg) | 650,- € | |
| DNA isolated from cell line (5 µg) | 160,- € | |
see price list