DERL-2
ACC 531
Cell line:
DERL-2
DSMZ no.:
ACC 531
Species:
human (Homo sapiens)
Cell type:
T cell lymphoma
Origin:
established from the peripheral blood of a 30-year-old man with hepatosplenic gamma-delta T cell lymphoma (T-cell NHL) in 1995 at disease progression; cells are IL-2 dependent; simultaneous sister cell line of cell line DERL-7 (ACC 524)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS + 20 ng/ml human IL-2
Subculture:
seed out at ca. 1.0 x 106 cells/ml, cells grow slowly at first, cultivate in 6- or 12-well-plates for optimal growth; a significant amount of cell debris may be present in the culture all the time; split saturated culture 1:2 every 2-3 days; maximal density of ca. 2.5 x 106 cells/ml; CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines.
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60 hours
Harvest:
cell harvest of ca. 2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture and PCR assays
Immunology:
CD2 +, CD3 -, CD4 -, CD5 -, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 8% polyploidy - 48(46-49)<2n>XY, +7, +8, +17, t(1;5)(p13;q32), i(7q), der(10;?17)(q10;q10) - sideline with +del(7)(p11) - i(7q) is associated with hepatosplenic T-NHL - matches originator's karyotype
Molec. Genetics:
expression of fusion gene CD53::PDGFRB confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list