CAKI-2
ACC 54
Cell line:
CAKI-2
DSMZ no.:
ACC 54
Species:
human (Homo sapiens)
Cell type:
kidney carcinoma
Origin:
established from the primary renal carcinoma of a 69-year-old man
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% McCoy's 5A + 10% h.i. FBS
Subculture:
change medium twice every week, split confluent cultures 1:2 to 1:3 weekly using trypsin/EDTA, upon thawing grow cells immediately in 80 cm2; seed out at ca. 2 x 106 cells/80 cm2 in 10 ml medium
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
cell harvest of ca. 1 x 106 cells/25 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded pentaploid/hexaploid karyotype with 12% polyploidy - 130(118-132)<6n>XXX, -Y, -Y, -Y, inc, add(1)(p22), i(8q), i(?5q)x2, del(6)(q23), t(14;15)(p10;q10), der(19)t(1;19)(q24;q13), dminx1, large iso der(5q)x2, and submeta marker der(?)t(?;2)(?;q11)x2 - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list