HT
ACC 567
Cell line:
HT
DSMZ no.:
ACC 567
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established from the ascitic effusion of a 70-year-old man with B cell non-Hodgkin lymphoma (B-NHL) in 1983 (diffuse large cell lymphoma); assigned to GCB-like lymphoma subtype (germinal center B-cell)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
85-90% RPMI 1640 + 10-15% h.i. FBS
Subculture:
seed out at ca. 0.3-0.5 x 106 cells/ml; split saturated culture 1:3 to 1:5 every 3-4 days; maintain at 0.5-1 x 106 cells/ml; max. density at about 1.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
cell harvest of ca. 1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD37 +, CD38 +, cyCD79a +, CD138 -, HLA-DR +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-diploid karyotype with 3% polyploidy - 46(42-46)<2n>XY, +2, der(2;4)(p10;q10), der(2)(del)(2)(p11?q21), dup(10)(q11q22-23), dup(11)(?q23qter) - no IGH-BCL2 rearrangement detected
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list