8709
ACC 588
Cell line:
8709
DSMZ no.:
ACC 588
Species:
murine (mouse) (Mus musculus)
Cell type:
acute myeloid leukemia
Origin:
established from the splenocytes of a radiation-induced acute myeloid leukemia (AML) of the C3H inbred mouse strain in 1997
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
cells growing singly or in small clusters in suspension, partly slightly adherent
Medium:
80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine + 10 ng/ml muIL-3 or 10-20% WEHI-3B supernatant
Subculture:
split culture 1:2 twice a week; seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.3-1.0 x 106 cells/ml; after thawing it is advisable to culture the cells first in 24-well plates and to add 10 ng/ml mIL-3 or 10-20% WEHI-3B supernatant daily; viability may be low after thawing
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60-80 hours
Harvest:
maximum density at about 1.2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Viruses:
PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list