BLUE-1
ACC 594
Cell line:
BLUE-1
DSMZ no.:
ACC 594
Species:
human (Homo sapiens)
Cell type:
Burkitt lymphoma
Origin:
established from the bone marrow of a 29-year-old man with Burkitt lymphoma in second relapse following combined chemotherapy and radiotherapy in 2004; cells are described in the literature to carry t(6;20)(q15;q11.2) leading to BACH2-BCL2L1 fusion gene
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
round cells growing singly or in clumps in suspension; image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/ml; maintain at 0.5 - 2.0 x 106 cells/ml; split 1:2 to 1:5 every 2-4 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
maximum density at about 3.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD13 -, CD19 +, CD20 -, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 -, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, cykappa -, cylambda +
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype; 53(49-53)<2n>XY, +6, +13, +16, +20, +20, +20, +21, t(6;20)(q15;q11.2), der(6)t(6;20)(q15;q11.2), t(8;14)(q24;q32), der(20)del(20)(p12.2p13.2)t(6;20)(q15;q11.2)t(6;11)(q16;p13)x2; carries t(8;14) effecting IGH-MYC juxtaposition; matches published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list