L-82
ACC 597
Cell line:
L-82
DSMZ no.:
ACC 597
Species:
human (Homo sapiens)
Cell type:
anaplastic large cell lymphoma
Origin:
established from the pleural effusion of a 24-year-old woman with recurrent anaplastic large cell lymphoma (ALCL); cells were described in the literature to clonally derive from T-lineage lymphoid cells and to be tumorigenic in SCID mice
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.3-0.5 x 106 cells/ml; split 1:2 to 1:4 every 2-3 days; maintain at ca. 0.3-1 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40 hours
Harvest:
maximum density at about 1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
cy/smCD3 -, CD4 +, CD5 +, CD6 -, CD7 -, CD8 -, CD11b -, CD13 -, CD14 -, CD15 -, CD19 -, CD20 -, CD25 +, CD30 +, CD34 -, CD71 +, TRCalpha/beta -, TCRgamma/delta -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded karyotype with 4% polyploidy
72-77<3n>XX/XXX,+1,+2,+5,+7,+9,+10,-14,-15,+4-6mar; add(X)(q12), add(X)(q27), add(1)(p11), t(2;5)(p23;q35), der(2)t(2;5)(p23;q35), der(4)add(4)(p14q35), der(6)i(6)(q10)t(1;6)(q41;p24), add(7)(q22), add(8)(q24), add(9)(p2?2)x2, add(9)(q11), add(10)(p1?), add(13)(q33), der(13)t(13;15)(p11;q14), del(16)(q23), add(17)(p1?);
carries t(2;5) effecting NPM1-ALK rearrangement, and 7q22 amplification targeting CDK6 upregulation;
matches published karyotype
Molec. Genetics:
expression of fusion gene NPM1-ALK confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list