PEER
ACC 6
Cell line:
PEER
DSMZ no.:
ACC 6
Species:
human (Homo sapiens)
Cell type:
T cell leukemia
Origin:
established from the peripheral blood of a 4-year-old girl with T cell acute lymphoblastic leukemia (T-ALL) in second relapse in 1977;
carries NUP214-ABL1 fusion gene; of note: PEER is genetically identical to cell line BE-13 (DSMZ ACC 396); however, PEER carries a diploid and BE-13 a tetraploid karyotype suggesting that BE-13 is a subclone of PEER, rather than vice-versa
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
cells are very difficult to culture! Cells grow at best in 24-well-plates; seed out at ca. 1.0 x 106 cells/ml (culture should not be split during the first 3-4 days after thawing), then maintain at ca. 0.5-1.0 x 106 cells/ml; split saturated culture 1:2 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-60 hours
Harvest:
saturation density at 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD2 -, CD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD13 -, CD19 -, CD34 -, TCRgamma/delta +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an
authentic profile of the reference STR database. Note: cell line BE-13 shows an identical STR profile
Cytogenetics:
human pseudodiploid karyotype with 7% polyploidy - 46(42-47)<2n>XX, der(4)?dup ins(4;4)(?p11;?q21q25), del(5)(q22q31), del(6)(q13q22), del(9)(p11p22), del(9)(q22) - resembles published karyotype - 5q-, 6q-, 9p- and 9q- associated with hematological malignancy
Molec. Genetics:
expression of NKX2-5 and MEF2C confirmed by RT-PCR and Western blot and expression of the fusion gene NUP214-ABL1 by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list