COS-7
ACC 60
Cell line:
COS-7
DSMZ no.:
ACC 60
Species:
monkey - African green monkey (Chlorocebus aethiops (Cercopithecus aethiops))
Cell type:
kidney
Origin:
derived from CV-1, a simian cell line (cercopithecus aethiops), by transformation with an origin-defective mutant of SV-40; cells were described in the literature to support the growth of SV-40 viruses; classified as risk category 1 according to the German Central Commission for Biological Safety (ZKBS)
Biosafety level:
1, GMO-S1
Risk assessment:
The cell line was established by transfection with a mutated simian virus 40 (SV40) exhibiting a 6 bp deletion in the replication origin. This renders the virus incompetent for replication and the cell line can thus be handled under biosafety level 1.
DSMZ Cell Culture Data:
Medium:
90% DMEM + 10% h.i. FBS
Subculture:
split confluent culture 1:3 to 1:5 every 2-3 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35-48 hours
Harvest:
about 2-3 x 106 cells/80 cm2; saturation density at about 5 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Chlorocebus aethiops
Cytogenetics:
simian hypodiploid karyotype with 25% polyploidy - 57(53-58)<2n>
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list