RS-5
ACC 604
Cell line:
RS-5
DSMZ no.:
ACC 604
Species:
human (Homo sapiens)
Cell type:
sarcomatoid mesothelioma
Origin:
established in 2003 from the untreated malignant mesothelioma of the pleura of a 78-year-old man with asbestos cancer
Reference(s):
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
90% NCTC-109 + 10% h.i. FBS + 2 mM L-glutamine
Subculture:
seed out at ca. 1-2 x 106 cells/175 cm2; split confluent culture 1:4 once a week using trypsin/EDTA; cells grow slowly for about one week after thawing
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 3 days
Harvest:
maximum density of ca. 3 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded near-diploid karyotype with 5% polyploidy; 46(39-48)<2n>XY; although no consistent abnormality was detected, most metaphases showed random chromosome loss
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list