Species:
human (Homo sapiens)
Cell type:
essential thrombocythemia
Origin:
established from the peripheral blood of a 71-year-old woman with essential thrombocythemia at megakaryoblastic leukemic transformation in 1995; cells carry the JAK2 V617F mutation and the DNMT3A R882H mutation. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Morphology:
mostly single cells in suspension, some cells grow loosely adherent, 1-3% giant cells;
image;
image;
image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
start and cultivate in 24- or 12-well plates for optimal growth; seed out at ca. 0,8 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; split culture 1:2 every 3-4 days; possibly no cell growth during first week after thawing
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60-80 hours
Harvest:
maximum density at ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD4 +, CD7 +, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, CD38 +, CD41 +, CD42 -,CD71 +, HLA-DR +;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded near-diploid karyotype with 8% polyploidy; 46-47<2n>XX, +13, -14, +21, +mar, add(4)(p15), der(5)t(5;19)(q11;q13), der(7)t(7;14)(q31;q11), del(9)(p11p24)ins(9;?21)(p11;p11p12), add(9)(q11)t(9;22)(p24;q12), der(13)del(13)(q11-q13)t(13;21)(q34;q11-12), der(13)t(13;21)(q34;q11-12), ins(16;9)(q22;?p23p24), der(19)t(9;19)(p24;q13), ider(?21;9)(p10p13;p24), der(21)r(21;9)(p1?1q1?1;p24p2?3);
carries multiple rearrangements effecting 9p24 amplification; resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
