R3327-MATLyLu
ACC 609
Cell line:
R3327-MATLyLu
DSMZ no.:
ACC 609
Species:
rat (Rattus norvegicus)
Cell type:
prostate tumor
Origin:
established from tumor material of a previously subcutaneously passaged rapidly growing, hormone independent, anaplastic and metastatic variant of the rat Dunning R 3327 AT prostate tumor in Copenhagen rats; cells were described in the literature to be androgen insensitive and to have a high metastatic potential with dissemination to lymph nodes and lungs in rats
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
polymorphic cells growing adherently in monolayers
Medium:
90-95% RPMI 1640 + 5-10% h.i. FBS
Subculture:
split culture 1:10 to 1:20 every two days using trypsin/EDTA; seed out at ca. 2 x 106 cells/175 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 15 hours
Harvest:
cell harvest of ca. 20 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Viruses:
PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list