GF-D8
ACC 615
Cell line:
GF-D8
DSMZ no.:
ACC 615
Species:
human (Homo sapiens)
Cell type:
acute myelocytic leukemia
Origin:
established from the peripheral blood of an 82-year-old man with acute myelocytic leukemia (AML M1) at diagnosis in 1989; described in the literature to be growth-dependent on granulocyte-macrophage colony-stimulating factor (GM-CSF) or interleukin-3 (IL-3)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
70% RPMI 1640 + 20% h.i. FBS + 10% vol conditioned medium of cell line 5637 (DSM ACC 35) (or 50 ng/ml of human recombinant GM-CSF)
Subculture:
seed out at ca. 0.5 x 106 cells/ml in 24-well plates; maintain at 0.3-1.0 x 106 cells/ml; optimal split ratio of 1:2 every 2-3 days; cells grow slowly for about one week after thawing. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines.
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48-72 hours
Harvest:
maximum density at ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 -, HLA-DR -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype; 49(45-51)<2n>XY, +8, +13, +21, +mar, der(5)t(5;15)(q11;q25), der(7)t(7;15)(q31;q24), ?inv(7)(q31.3q35)x2, der(8)ins(8;11)(q24;q23q24)dup(8)(q23q24)dup(11)(q22q23), der(11)t(8;11)(q24;q23)dup(8)(q23q24)dup(11)(q22q23), der(11;17)(q10;q10), add(12)(p13), der(14)t(5;14)(q32;q24); resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list