CL-50IIa
ACC 623
Cell line:
CL-50IIa
DSMZ no.:
ACC 623
Species:
rat (Rattus norvegicus)
Cell type:
cholangio-hepatocellular tumor
Origin:
established ca. 1986 from the liver of a diethylnitrosamine-treated male Wistar AF/Han rat
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
epitheloid cells growing adherently in monolayers
Medium:
80-90% MEM (with Earle's salts) + 10-20% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/80 cm2; optimal split ratio 1:3 every 3-4 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-60 hours
Harvest:
maximum density at ca. 6 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Viruses:
PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list