BL-2
ACC 625
Cell line:
BL-2
DSMZ no.:
ACC 625
Species:
human (Homo sapiens)
Cell type:
Burkitt lymphoma
Origin:
established in 1979 from the bone marrow of a 7-year-old boy with non-endemic Burkitt lymphoma (stage III) after chemo- and radiotherapy; cells are described in the literature to express no Epstein-Barr virus (EBV) antigen and that somatic immunoglobulin gene hypermutation can be induced; a paired EBV+ B-lymphoblastoid cell line (B-LCL, IARC-304) is available (DSMZ ACC 897)
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; optimal split ratio 1:3 to 1:5 every 2-4 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 24 hours
Harvest:
maximum density at ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded near-diploid karyotype with 3% polyploidy; 44-47<2n>XY, der(1)t(1;7)(q32;q11.2), der(6)t(1;6)(q21;q25), t(8;22)(q24;q11.2), del(11)(q24.2); carries t(8;22) effecting juxtaposition of MYC with IGL; matches published karyotype
Molec. Genetics:
IGL::MYC translocation was verified by genomic PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list