ULA

human (Homo sapiens)

B cell lymphoma

established in 2002 from the mononuclear ascites cells of a 57-year-old man with terminal diffuse large B cell lymphoma (stage IV) after failing to respond to several rounds of chemotherapy (CHOP, ICE)

1

A

Transfer to third parties needs to be negotiated with the depositor.

round cells growing as single cells or in small clumps in suspension or slightly adherent; when cultured on extracellular matrix (Matrigel) cells grow adherently; image; image

80% Iscove´s MDM + Optimem (at 1:1) + 20% h.i. FBS

seed out at ca. 0.5 x 10⁶ cells/ml; optimal split ratio 1:2 to 1:5 every 2-3 days; to increase viability flasks may be coated with Matrigel (dilute 1:3 - 1:5 in Optimem, incubate for 2 hours at room temperature, then carefully remove excess medium and wash two times gently with PBS)

at 37 °C with 5% CO₂

ca. 40-60 hours

maximum density at ca. 1 x 10⁶ cells/ml

frozen with 70% medium, 20% FBS, 10% DMSO

negative in microbiological culture, PCR assays

CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

human hyperdiploid karyotype with 8% polyploidy 47(42-48)<2n>XY, +7, +8, -15, der(3)del(3)(p21p22)t(3;8)(q27;q24.1), t(3;8)(q27;q24.3), t(4;20)(q21;p12), t(7;12)(q22;p13), der(8)t(3;8)(q27;q24.3), der(8)t(3;8)(q27;q23)t(8;7)(q24.3;q11), inv(9)(p21q34), del(10)(q22), t(14;18)(q32;q21), del(19)(q13.3); sdl with t(4;20); carries t(14;18) effecting IGH-BCL2 rearrangement; resembles published karyotype

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Delivery form		Prices
Frozen culture		450,- €
Growing culture   (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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