DOGKIT
ACC 629
Cell line:
DOGKIT
DSMZ no.:
ACC 629
Species:
human (Homo sapiens)
Cell type:
Burkitt lymphoma
Origin:
established from the peripheral blood of a 56-year-old man with EBV-negative Burkitt lymphoma after second relapse; described in the literature to carry t(8;22) and t(14;18) associated with MYC and BCL2 rearrangements, respectively
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
round cells growing singly in suspension; image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at 0.1-1.0 x 106 cells/ml; optimal split ratio 1:2 to 1:8 every 2-4 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-50 hours
Harvest:
maximum density at ca. 2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD38 +, cyCD79a +, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hyperdiploid karyotype with 3.5% polyploidy; 46-49<2n>XY, +20, dup(1)(q21q42), der(2)t(2;14)(q37;q24)t(14;18)(q32;q21), t(8;22)(q24;q11.2), der(14)t(X;14)(p21;p13)t(14;18)(q32;q21), dup(17)(q11q22), der(18)t(14;18)(q32;q21); supernumerary acf/dm present at 14%; sdl with add(16)(p13); resembles published karyotype; carries t(8;22) and t(14;18) rearrangements effecting IGL-MYC and IGH-BCL2 rearrangements, respectively
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list