GUMBUS
ACC 630
Cell line:
GUMBUS
DSMZ no.:
ACC 630
Species:
human (Homo sapiens)
Cell type:
Burkitt lymphoma
Origin:
established from the liquor of a 28-year-old man with Burkitt lymphoma at second relapse that became highly resistant to several chemotherapy regimens
Reference(s):
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
small round cells growing in clumps in suspension; image
Medium:
95% RPMI 1640 + 5% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5 x 106 cells/ml; optimal split ratio 1:2 to 1:3 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48 hours
Harvest:
maximum density at ca. 2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near diploid karyotype with 4% polyploidy
46(44-46)<2n>,XY, del(3)(p11p21.2), del(6)(q14q24), t(8;14)(q24;q32); carries t(8;14) effecting rearrangement of MYC with IGH; resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list