MB-1
ACC 638
Cell line:
MB-1
DSMZ no.:
ACC 638
Species:
human (Homo sapiens)
Cell type:
anaplastic thyroid carcinoma
Origin:
established in 2007 from the large retrosternal mass resected before treatment from a 57-year-old man with rapidly fatal anaplastic thyroid cancer
Reference(s):
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
round epitheloid cells growing in monolayers
Medium:
80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine
Subculture:
seed out at ca. 1-3 x 106 cells/175 cm2; optimal split ratio of 1:3 to 1:10 every 3-7 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60-100 hours
Harvest:
cell harvest of ca. 10-15 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list