NT-92
ACC 639
Cell line:
NT-92
DSMZ no.:
ACC 639
Species:
rat (Rattus norvegicus)
Cell type:
cholangio-hepatocellular tumor
Origin:
established ca. 1986 from the cholangio-hepatocellular tumor induced in vivo by treatment of a male Wistar AF-Han rat with a single injection of N-methyl-N-nitrosourea and oral administration of phenobarbital
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
epitheloid cells growing adherently in monolayers
Medium:
90% MEM (with Earle's salts) + 10% h.i. FBS
Subculture:
seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:2 to 1:4 every 4 days using trypsin/EDTA for 10 min
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 3-4 days
Harvest:
cell harvest of ca. 15 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Viruses:
SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list