ST
ACC 641
Cell line:
ST
DSMZ no.:
ACC 641
Species:
porcine (pig) (Sus scrofa)
Cell type:
testis
Origin:
established from trypsinized testicular tissue of a porcine fetus
Reference(s):
Biosafety level:
1
Risk assessment:
The cell line carries Porcine type-C oncovirus (PCOV) sequences and their transcripts, which were detected by PCR and RT-PCR, respectively. We did not determine the infection mode of these viruses and cannot exclude the secretion of the viruses. In Germany, these viruses are classified BSL 1 regarding men and BSL 2 relating to animals (TRBA 462). However, the German Central Committee on Biological Safety (ZKBS) classified these viruses and infected cell lines BSL 2 when they are used for genetically modifying applications (Ref. 6790-10-65).
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
fibroblastoid cells growing in monolayers
Medium:
90% MEM (with Earle's salts) + 10% h.i. FBS + 1 mM sodium pyruvate + non-essential amino acids
Subculture:
seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:5 - 1:10 every 3-4 days using trypsin/EDTA (for more than 10 minutes)
Incubation:
at 37 °C with CO2
Doubling time:
ca. 30-40 hours
Harvest:
cell harvest of ca. 4-5 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Sus scrofa species.
Viruses:
SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list