U-2973
ACC 642
Cell line:
U-2973
DSMZ no.:
ACC 642
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established in 2006 from the peripheral blood of a 42-year-old man with mature B-cell leukemia; described in the literature to carry t(14;18)(q32;q21) with IGH-BCL2 and biallelic MYC rearrangement with non-IG partner
Reference(s):
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
round cells growing singly in suspension; image
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at 0.3-2.0 x106 cells/ml, optimal split ratio of 1:3 to 1:6 every 3-5 days; viability may drop below 50% within the first 3-4 days after thawing; we recommend to start culture with a cell concentration of 1.0 x 106 cells/ml and to cultivate the cells initially in 24-well-plates in the presence of 20% FBS
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35 hours
Harvest:
maximum densitiy of ca. 3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD38 +, cyCD79a +, CD138 +, HLA-DR +, sm/cyIgG +, sm/cyIgM -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 4% polyploidy; 47(44-48)XY, +12, del(6)(q24.2q26), t(8;12)(q24;p12)x2, t(14;18)(q32;q21), der(20)t(18;20;q21;q13); t(14;18) effects rearrangement of IGH and BCL2; t(8;12) effects non-IG, MYC rearrangement; resembles published karyotype
Molec. Genetics:
expression of fusion gene IGH-BCL2 confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list